Recent advances in microfluidic organ-on-a-chip technology have enabled the development of 3D microphysiological systems for various biological studies. However, traditional PDMS-based microfluidic platforms have limitations in fabrication and usage, creating a need for more accessible, standardized, and mass-producible platforms. Introducing the Injection-Molded Plastic Array 3D Culture (IMPACT) platform, a novel microfluidic system designed for easy and diverse patterning of 3D cellular hydrogel. The IMPACT platform allows high-content morphological profiling of nine different tumor cells’ effects on vascular formation and screening of three anti-tumor drugs (5-FU, axitinib, cetuximab) at various dosages. Distinct molecular mechanism-dependent responses were observed, confirming the platform’s potential as a high-content drug testing tool. IMPACT represents the next generation of 3D microfluidic co-culture platforms, suitable for biological, clinical, and pharmaceutical research requiring high-throughput and high-content assays.-Scientific Journal cover design by scapiens

https://pubs.rsc.org/en/content/articlelanding/2019/lc/c9lc00148d#!divAbstract